Maximum memory used for sorting can be specified using -m with an M or G suffix with a default of 2G. This will create a new BUS file where the BUS records are sorted by barcode first, UMI second, and equivalence class third. ![]() ![]() Required if using -p, otherwise defaults to output T, -temp Location and prefix for temporary files To simply and accelerate downstream processing BUS files can be sorted using bustools sort Raw BUS output from pseudoalignment programs may be unsorted. Note that in many cases using -p will write a binary file to stdout which may garble your terminal unless parsed by a subsequent bustools command. Many of the bustools commands can read from the standard input ( stdin), by specifying - as the input file and write to standard output ( stdout) using the -p flag if available. Running bustools without arguments prints usage information for Whitelist Generate a whitelist from a BUS file Text Convert a binary BUS file to a tab-delimited text file ![]() Sort Sort a BUS file by barcodes and UMIs Linker Remove section of barcodes in BUS files Inspect Produce a report summarizing a BUS file Count Generate count matrices from a BUS file
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